mouse anti dopamine receptor d2  (Santa Cruz Biotechnology)

Journal: Theranostics

Article Title: Dopamine D2 receptor modulating mPFC-BLA circuit contributes to chronic sleep deprivation-induced memory impairment in mice

doi: 10.7150/thno.114797

Figure Lengend Snippet: Chronic sleep deprivation increased dopamine D2 receptor gene expression in the mPFC. (A) Experimental design. SD, sleep deprivation; S, sleep. (B) Volcano plot of mPFC gene expression after CSD. padj, adjusted p-value; ns, no significance. (C) Heatmaps showing differentially expressed genes in the Con and CSD groups. Con, the control group; CSD, the chronic sleep deprivation group. (D) Quantification of Drd2 , Npase4 , and Baiap3 gene expression between the two groups. (E) Gene ontology functional analysis diagram for the CSD group. (F) Heatmaps showing the gene expression of GABA receptor subunits ( Gabrs ) in the Con and CSD groups. (G) Heatmaps showing the correlation of Drd2, Npase4, and Gabrs. *p < 0.05. n = 4 mice for each group.

Article Snippet: Slices were washed three times with PBS, each wash lasting 10 min, then blocked with immunofluorescence blocking solution (P0260, Beyotime Biotechnology Co., Shanghai, China) for 1 h. The blocking solution was washed off with three 10-minute PBS washes and incubated overnight at 4 °C with primary antibodies: mouse anti-Dopamine D2 receptor (1:200; sc-5303, Santa Cruz), rabbit anti-CaMKIIα (1:500; ab5683, Abcam), or guinea pig anti-c-Fos (1:500; 226308, synaptic system).

Techniques: Gene Expression, Control, Functional Assay

Journal: Toxicological Research

Article Title: Influence of Lead on Repetitive Behavior and Dopamine Metabolism in a Mouse Model of Iron Overload

doi: 10.5487/tr.2014.30.4.267

Figure Lengend Snippet: Fig. 6. Effect of Pb exposure via drinking water on dopamine-related protein expression in the brain of Hfe-mutant mice. Western blot analysis was performed to analyze expression levels of tyrosine hydroxylase (A), dopamine transporter (B), dopamine D1 receptor (C) and dopamine D2 receptor (D) in the brain from mice exposed to Pb at 1.6 mg/ml by drinking water (n = 4~8 per group). Open and closed bars represent water and Pb treatment, respectively. All data were presented as mean ± SEM. All the P values were calcu- lated by two-way ANOVA, followed by post-hoc comparisons. WT, wild-type; MUT, Hfe-mutant.

Article Snippet: The antibodies used were rabbit antibody against dopamine D1 receptor (D1DR, 1:500, Abcam; Cambridge, MA, USA), mouse antibody against dopamine D2 receptor (D2DR, 1 : 100, Santa Cruz Biotech, Dallas, Texas), goat antibody against dopamine transporter (DAT, 1 : 200, Santa Cruz Biotech), mouse antibody against tyrosine hydroxylase (TH, 1 : 200, Santa Cruz Biotech) or rabbit antibody against ferritin (Ftn, 1 : 500, Santa Cruz Biotech).

Techniques: Expressing, Mutagenesis, Western Blot